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Registros recuperados: 43 | |
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Arnaud, Sophie; Goyard, Emmanuel; Prou, Jean; Vonau, Vincent; Bonhomme, Francois; Boudry, Pierre. |
Exploitation of the pearl oyster Pinetada margaritifera, which has developed in French Polynesia over the last twenty years, is based mainly on the collection of natural spat. Recruitment is highly variable in space and time and so spat is very often transferred by the farmers between atolls or even between archipelagos. In previous studies, anonymous nuclear markers demonstrated that This development has coincided with a genetic homogenisation of the wild stocks since the 1980s. This suggests a high level of reproductive success on farms, which is likely to be due to the high density of animaIs when compared to the density in the wild. We used the same markers to show that the level of genetic variability observed on farms is not significantly different... |
Tipo: Text |
Palavras-chave: French Polynesia; Genetic diversity; Spat; Pinctada margaritifera; Pearl oyster; Polynésie française; Diversité génétique; Naissain; Pinctada margaritifera; Huître perlière. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3822.PDF |
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Arnaud, Sophie; Goyard, Emmanuel; Vonau, Vincent; Herbaut, C; Prou, Jean; Saulnier, Denis. |
Most bivalves species of the genus Pinctada are well known throughout the world for production of white or black pearls of high commercial value. For cultured pearl production, a mantle allograft from a donor is implanted into the gonad of a recipient oyster, together with a small inorganic bead. Because of the dedifferentiation of cells during the first steps of the host oyster's immunological reaction, so far the fate of the graft and its exact role in the process of pearl formation could not be determined via classical histological methods. Here we report the first molecular evidence of the resilience of the graft in the recipient organism by showing that cells containing genome from the donor are still present at the end of pearl formation. These... |
Tipo: Text |
Palavras-chave: Genotype; Pearl; Pearl sac; Grafting; Biomineralization; Pearl oyster. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2456.pdf |
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Arnaud, Sophie; Vonau, Vincent; Rouxel, Catherine; Bonhomme, Francois; Prou, Jean; Goyard, Emmanuel; Boudry, Pierre. |
In order to study further the genetic structure of the pearl oyster Pinctada margaritifera in French Polynesia with a special consideration for the sampling scale, we analyzed or re-analyzed sets of data based on nuclear DNA markers obtained at different spatial scales. At a large scale (several 1,000 km), the remote Marquesas Islands were confirmed to be significantly differentiated from Tuamotu-Gambier and Society archipelagos, with a marked difference however for the two main islands that are different from each other. At a medium scale (several 10 to several 100 km), overall homogeneity was observed within and between these two archipelagos, with some exceptions. This could be attributed both to large-scale larval dispersal and to human-driven spat... |
Tipo: Text |
Palavras-chave: Population genetics; Polynesia; Bivalve; Pearl oyster; Benthic organisms; Sampling strategy; Genetic patchiness. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4960.pdf |
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Blay, Carole; Planes, Serge; Ky, Chin-long. |
Grafting mantle tissue of a donor pearl oyster into the gonad of a recipient oyster results in the formation of a chimera, the pearl sac. The phenotypic variations of this chimera are hypothesized to be the result of interactions between the donor and recipient genomes. In this study, the heritability of phenotypic variation and its association with gene expression were investigated for the first time during P. margaritifera pearl production. Genetic variance was evaluated at different levels, 1) before the graft operation (expression in graft tissue), 2) after grafting (pearl sac tissue expression in chimera) and 3) on the product of the graft (pearl phenotype traits) based on controlled bi-parental crosses and the F1 generation. Donor related genetic... |
Tipo: Text |
Palavras-chave: Gene expression; Heritability; Pearl oyster; Phenotype; Pinctada margaritifera. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00433/54505/55876.pdf |
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Buestel, Dominique; Pouvreau, Stephane. |
Results of research concerning the food of the pearl oyster Pinctada mai margaritifera are presented, by taking Takapoto atoll (Tuamotu archipelago, French Polynesia) as a study site. Monthly sampling of several parameters representative of bivalve environment and food (water temperature, salinity, mineral and organic matter, proteins, lipids and carbohydrates, chlorophyll a and phaeopigments) confirm and increase knowledge of the Takapoto lagoon hydrobiology. The variability of these parameters was analysed. Takapoto lagoon showed a remarkable spatial stability. Temporal variations showed a low seasonal trend. Local meteorology (wind, precipitation) had an influence on short-term variability (especially for mineral matter). Night and day rhythm also... |
Tipo: Text |
Palavras-chave: Pinctada margaritifera; Matière en suspension; Capacité trophique; Huı̂tre perlière; Atoll; Pinctada margaritifera; Suspended matter; Carrying capacity; Pearl oyster; Atoll. |
Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00000/10529/7078.pdf |
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Buestel, Dominique; Pouvreau, Stephane; Tiapari, Jerome; Bougrier, Serge; Chabirand, Jean-michel; Geairon, Philippe; Fougerouse, A. |
A study of pearl oysters Pinctada margaritifera growth and mortality was done on a monthly basis between June 1990 and September 1991 in Takapoto atoll (Tuamotu archipelago). Meanwhile, sampling parameters representative of bivalves food showed a remarquable spatial stability whithin the lagoon. The amplitude of temporal variability varied in the order of some days. Carrying capacity appears low : energetic content of particulate material in waters is about 2 joules/liter, ten times lower than in temperate areas. On the opposite, seasonal effect is reduced and particulate food is always available. Oysters rearing conditions were appropriate during the studied period with a low mortality rate and a regular growth in size (from 97 mm to 136 mm). Pearls... |
Tipo: Text |
Palavras-chave: Atoll; Pearl oyster; Carrying capacity; Growth; Pinctada margaritifera; Atoll; Huître perlière; Capacité Trophique; Croissance; Pinctada margaritifera. |
Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/1995/rapport-1642.pdf |
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Cochennec-laureau, Nathalie; Montagnani, Caroline; Saulnier, Denis; Fougerouse, Angelique; Levy, Peva; Lo, Cedrik. |
Pearl oyster grafting is a complex surgical operation that should lead to pearl formation after approximately eighteen months. Although this technique has been used for many years in French Polynesia, the grafting process is still not standardised. While studies have been carried out in order to improve graft performance and yield, these remain highly variable due to post-grafting mortality, nucleus rejection and unreliable pearl quality, all of which constrain pearl farm profitability. The present study uses histological analysis to monitor oysters that either rejected or retained their nuclei. Both groups of oysters are compared in terms of evolution of the graft, which could influence retention, and the development of a pearl sac in cases where grafting... |
Tipo: Text |
Palavras-chave: Grafting process; Pearl formation; Pearl oyster; Pteriidae; Pinctada margaritifera. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11288/7823.pdf |
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Dubé, Caroline; Ky, Chin-long; Planes, Serge. |
Elucidating the role of prokaryotic symbionts in mediating host physiology has emerged as an important area of research. Since oysters are the world’s most heavily cultivated bivalve molluscs, numerous studies have applied molecular techniques to understand the taxonomic and functional diversity of their associated bacteria. Here, we expand on this research by assessing the composition and putative functional profiles of prokaryotic communities from different organs/compartments of the black-lipped pearl oyster Pinctada margaritifera, a commercially important shellfish valued for cultured pearl production in the Pacific region. Seven tissues, in addition to mucous secretions, were targeted from P. margaritifera individuals: the gill, gonad, byssus gland,... |
Tipo: Text |
Palavras-chave: Pearl oyster; Microbiome; Tissue-specific bacterial communities; 16S rRNA gene sequencing; Functional profiling prediction. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00505/61670/65589.pdf |
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Gueguen, Yannick; Czorlich, Yann; Mastail, Max; Le Tohic, Bruno; Defay, Didier; Lyonnard, Pierre; Marigliano, Damien; Gauthier, Jean-pierre; Bari, Hubert; Lo, Cedrik; Chabrier, Sebastien; Le Moullac, Gilles. |
Cultured pearls are human creations formed by inserting a nucleus and a small piece of mantle tissue into a living shelled mollusc, usually a pearl oyster. Although many pearl observations intuitively suggest a possible rotation of the nucleated pearl inside the oyster, no experimental demonstration of such a movement has ever been done. This can be explained by the difficulty of observation of such a phenomenon in the tissues of a living animal. To investigate this question of pearl rotation, a magnetometer system was specifically engineered to register magnetic field variations with magnetic sensors from movements of a magnetic nucleus inserted in the pearl oyster. We demonstrated that a continuous movement of the nucleus inside the oyster starts after a... |
Tipo: Text |
Palavras-chave: Pearl oyster; Pearl rotation; Biomineralization. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38598/37127.pdf |
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Gueguen, Yannick; Montagnani, Caroline; Joubert, Caroline; Marie, Benjamin; Belliard, Corinne; Tayale, Alexandre; Fievet, Julie; Levy, Peva; Piquemal, David; Marin, Frederic; Le Moullac, Gilles; Ky, Chin-long; Garen, Pierre; Lo, Cedrick; Saulnier, Denis. |
Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding the molecular mechanisms underlying biomineralization processes... |
Tipo: Text |
Palavras-chave: Pearl oyster; Biomineralization; Pinctada margaritifera. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00167/27840/26049.pdf |
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Hui, Belinda; Vonau, Vincent; Moriceau, Jacques; Tetumu, Roger; Vanaa, Vincent; Demoy-schneider, Marina; Suquet, Marc; Le Moullac, Gilles. |
Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the development of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry. Spermatozoa were cryopreserved with CPA 0.7 M trehalose in 0.8 M Me2SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and stored for one week before use. The viability of thawed sperm was 23 % lower than that of fresh sperm. When using thawed... |
Tipo: Text |
Palavras-chave: Cryopreservation; Spermatozoa; Fertility; Larval rearing; Pearl oyster; Pinctada margaritifera. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00040/15098/12446.pdf |
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Registros recuperados: 43 | |
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